Luminometer Transport Drive Alignment Procedure

Parts and Materials Required

• Proper PPE
• Spare MTUs
• Syscheck Kit (if necessary)

Time Required

• 60 minutes (if Syscheck is necessary)
• 3 hours (if teaching is necessary)

Procedure

1. Put on proper PPE.
2. Power off the Panther System, but leave the PC on.
3. Disconnect the PMTPhotomultiplier tube ribbon cable from the Luminometer PCB. If necessary, safely open the Service Drawer.

   

4. Power on the Panther System.
5. Login to the FSE Shield and start Service Software.
6. Load at least one MTUMulti-tube unit—Container used to process tests in the instrument. An MTU contains five separate reaction tubes. The MTU is moved through the instrument by the linear distributor and includes five tiplets for pipettiing to be used in the mag wash station. into the Input Queue.
7. From the 1. Distributor tab, select Main. Initialize the following modules:
   1. Select Distributor from the Common Control dropdown menu and click Initialize.
   2. Select Front and Loading Units from the dropdown menu and click Initialize.
   3. Select Luminometer from the dropdown menu and click Initialize.

      

8. Place an MTU into the Luminometer:
   1. From the 1. Distributor tab, select Move.
   2. Open the Luminometer door by clicking Open.
   3. Click Push MTU (ensure at least one MTU is loaded in the Input Queue).
   4. Double-click Input Queue on the Distributor Single Movement List to pick an MTU.
   5. Double-click Luminometer to place the MTU.
   6. Click Close to close the Luminometer door.

   

9. Open the Service Drawer.
10. Remove the Luminometer top cover:
    1. With the Service Drawer fully open remove the 5 screws that are visible (3mm hex driver).

       

    2. With the Service Drawer about half-way closed, remove the other 5 screws that can be seen between the TCRTarget capture reagent—An assay-specific reagent added as part of specimen pipetting. Carousel and the peristaltic pump.

       

    3. Carefully move the Luminometer cover aside and rest it on the Chiller Ramp. Do not strain the cables that are attached underneath the lid.

       

11. From the 6. Luminometer tab, select Parameter. Enter 1 in the Authorization Code field and click Set.

    

12. Select Teach. Click the Get button in the “Move axis to Position/Get Stored Teach Position” section on the bottom right of the screen.

    Note—Left / Right value should be between 1270-1320.

13. Click the Move button.

    

14. The transport drive will move Tube 1 of the MTU above the injection location.

    Note—The tab on side of Tube 1 (tube at the back, near the barcode) should be lined up inside the cut out of the Lumo frame. The tab does not need to be exactly in the center.

15. Check the shutter alignment.

    Note—Watch the MTU when moving the shutter. The MTU should not move while the shutter is opening or closing.

    1. Select the Move Shutter Up from the “Options for selected Device and Drive” menu and click Execute.
    2. Select the Move Shutter Down and click Execute. Repeat to evaluate movement.
    3. The MTU should not move during shutter movement. Even slight movement of the MTU means the MTU needs to be correctly aligned over the shutter.
    4. If there is any MTU movement, proceed to Step 16 and align the Transport Drive. Otherwise, proceed to Step 17.

       

16. Align the Transport Drive.

    Note—You will only teach to Tube 1, all remaining tubes will be calculated.

    1. Click on the right and left arrows in the “Incremental Positioning” section to move the MTU tab closer to the center of the cutout.

       

    2. Save the coordinate by clicking the Save button under the “Save Current Axis Positions/Get Current Pos.”

       

    3. Go back to Step 14 to check the shutter alignment again.
17. If no alignment was necessary, proceed to the Verification section. If teaching coordinates were changed, save the new position. Under “Options for selected Device and Drive”:
    1. Select Overwrite NVM then click Execute. (Select Yes at all prompts.)
    2. Select Calculate and Set other Tube Positions then click Execute.
    3. Select Overwrite NVM then click Execute.

       

Verification

1. With the Luminometer cover off, verify all five tube positions on the MTU are aligned properly with the shutter:
1. From the 6. Luminometer tab, select Main.
2. Under “Move MTU Drive”, select Tube No. 1 and then Measure. Verify the MTU does not move during shutter movement.
3. Repeat Step 1b. for all 5 MTU positions. (Select Tube No. 2 and then Measure, etc.)
4. If the shutter is moving freely, go to Step 2. Otherwise go back to Steps 15-17 in the Procedure section and align the shutter again.

   

2. Unload the MTU in the Luminometer:
   1. Select Tube No.1 and click Unload.

      

   2. From the 1. Distributor tab, select Move.
   3. Double-click Luminometer on the Distributor Single Movement List to pick the MTU.
   4. Double-click Service Queue to discard the MTU.

      

3. Re-install the luminometer cover:
   1. With the drawer fully open finger tighten the first set of 5 screws all the way down to the lid.
   2. With the drawer partially closed finger tighten down the second set of 5 screws down to the lid.
   3. Now with the 3 mm screw driver tighten down all the 10 screws. You will need to fully open and partially close the drawer to get to all the screws.
4. Power down the Panther System.
5. Reconnect the PMT ribbon cable to the Luminometer PCB.
6. Close the Service Drawer.
7. Perform the following procedures:
   1. Lumo OQ using Service Software
   2. Darkcount test using Service Software
8. If teaching coordinates were changed in the Procedure, Steps 14-16, perform the following procedures:
   1. Perform a Panther Luminometer Syscheck Reagent Verification procedure.