Additional Parking Slots Installation

Purpose

The purpose of this procedure is to provide instructions on additional Fusion installation requirements, including the Additional Parking Slots, Thermocycler Fiber Cleaning Nests, and the location of the CAN cables for the Panther Waste on the Fly and Fusion Sidecar on the Panther COP PCB for Panther Fusion Plus Systems.

What is Affected

This procedure provides instruction on how to install Additional Parking Slots to buffer Panther Waste on the Fly for a Panther Fusion Plus System.

Waste on the Fly allows the Fusion to continue processing, while the Panther’s Waste drawer is opened, by buffering MTUs in the Output Queue until the drawer is closed.

The correct location of the Panther Plus Waste on the Fly CAN cable on the Panther COP PCB.

Must be running System SW v7.2 or higher and Service Software v5.0.22.0 or higher.

Parts and Materials Required

Time Required

  • Hardware Installation – 15 Minutes if Thermocycler Fiber Cleaning Nests previously installed or 45 minutes for both Additional Parking Slots and Fiber Cleaning Nests

Procedure

Part A – CAN Cable Connections on the Panther COP PCB

  1. For existing Panther Fusion upgrading to Panther Fusion Plus: If CAN Bus Terminator is installed in position J12 it will be removed and the Fusion CAN cable moved to position J12 (the terminator can be discarded). The Panther Plus Waste on the Fly CAN cable will be plugged into position J11.

  2. For new or existing Panther upgrading to Panther Fusion Plus the CAN Bus Terminator will be removed (if present) and the CAN connections for WOTF and Fusion will be as in A.i above.

  3. For Panther Plus upgrading to Panther Fusion Plus the ClosedCAN Bus Terminator will be removed from J12 and the Fusion CAN will replace it in J12.

Part B – Thermocycler Fiber Cleaning Nests

  1. IF NOT previously installed, refer to Thermocycler Fiber Cleaner Nest Installation.

Part C – Fusion Additional Parking Slots

  1. Power down the Panther System.

  2. Disconnect the ClosedCAN cable from Cartridge Carousel.

  3. Remove routing of the ClosedCAN cable through the Fusion Waste Chute to allow Parking Slots to be mounted, as shown below.

  4. Remove the ClosedFusion Service Queue and save the 2 mounting screws.

  5. Route the ClosedCAN cable through hole in the front right of the Additional Parking Slots, then through the existing frame routing holes of the Fusion Waste Chute.

  6. Re-route and reconnect the ClosedCAN cable to the Cartridge Carousel.

  7. Mount the ClosedAdditional Parking Slots using the 2 screws (removed earlier) where the Service Queue was previously located.

  8. Perform Instrument Setup, and select all options that apply.

  9. Perform a Full Rotary distributor teaching.

  10. IF Fiber Cleaning Nests were newly installed, perform Fusion Vial Robot teaching to the Nests.

Verification

  1. Perform a 2 cycle OQ of the Additional Parking Slots on the Fusion Rotary Distributor.
  2. Install the Fiber Cleaning Trays. Refer to Part D of Thermocycler Fiber Cleaner Nest Installation.
Note—Informing the Panther software of loaded trays is not a customer accessible option and can only be done with the service login. It must be performed at install/upgrade of 7.2 software. Refer to TB-01772 Panther, Panther Fusion, Panther Plus and Panther Pooling Installation of System and AssayClosedProcedures required to prepare and perform a specific test. In the context of this document, assay refers exclusively to a Hologic test, such as Aptima Combo or Ultrio. Software v7.2.

Additional Information

After Instrument Setup is complete, the Fusion COP, Parameter 64 dictates the configuration of the Fusion Pipettors and Additional Parking Slots. The following table describes four possible configurations:

  Fusion APP Pipettor Fusion BLDC Pipettor
Without Additional Parking Slots 1 3
With Additional Parking Slots 9 11