Unloading Undeactivated MTUs from Amp and HPA Load Station

Purpose

To explain the method for removing and deactivating MTUs from the Amp and HPAHybridization protection assay—The Hologic HPA technique uses a specific DNA probe, labeled with an acridinium ester detector molecule that emits a chemiluminescent signal. Load Station in the event of a failure.

Parts and Materials Required

• Proper PPE
• Panther Tool Kit
• Squirt Bottle with recommended sodium hypochlorite concentration
  • Procleix Assays: 0.5 to 0.7%
  • AptimaAutomated Probe Transcription Mediated Amplification. Trade name for amplified NAT infectious disease assays and reagents designed for use with Hologic's instrument platforms. and other diagnostic assays: 2.5 to 3.5%
• Absorbent Bench Pads
• Hex Wrench Set

Procedure

1. Put on proper PPE.
2. Power down the Panther System.
3. Dispose of MTUMulti-tube unit—Container used to process tests in the instrument. An MTU contains five separate reaction tubes. The MTU is moved through the instrument by the linear distributor and includes five tiplets for pipettiing to be used in the mag wash station. waste bag and replace with a new waste bag.
4. Change Gloves.
5. Open the right and left side panel doors.
6. Verify that both pipettor arms are up. Manually push them up if they are not.
7. Remove the Luminometer injectors by unscrewing the two screws that secure the injectors to the luminometer module.

   

8. Unlock the Mid Bay drawers (left and right side) and pull the Mid Bay all the way out.
9. Slide the load station door to the left to access MTU.

   

10. Pull the MTU slowly out of the load station.

    

11. Bleach each tube of the MTU as it is being pulled out of the load station.

    

12. Gently place MTU into the waste bin.
13. Wait 15 minutes and then dispose the waste bag in a bio-hazard waste container.