MagWash Aspiration Tubing Replacement Procedure

Parts and Materials Required

• Proper PPE

• Panther Tool Kit
• Aspiration Tubing (Set of 5)

Time Required

• 20 Minutes per Set

Diagnosis

1. Systems that are covered by this procedure exhibit the following:
   1. The system has MagWash vacuum aspiration tubing held in place with black-oxide colored metal spring clips as shown below.

      

   2. The system has reported ML2 (MagWash Wash 1/2 wash buffer level too high) or ML5 (too much residual wash detected) flags, and investigation has eliminated clogged MagWash aspirators, a faulty Wash dispense pump or any other reason as a cause.
   3. They system has reported MagWash errors during prime and investigation has eliminated clogged MagWash aspirators, a faulty Wash dispense pump or any other reason as a cause.
   4. A visual inspection shows kinked or damaged tubing.

Procedure

Note—Panther System power may remain on during this procedure; however, the system must be idle.

Warning—This procedure involves manipulating waste tubing that may contain contaminated material. Take extra care not to expose yourself or the system to contaminates that may be withing the waste tubing.

1. Put on proper PPE.

2. Open the Left and Right Canopy Door.
3. Remove the partition wall between the Sample Bay and the Tip Drawers.
4. Place absorbent pads on top of the Sample Bay and Tip Drawers.
5. Firmly grasp the metal spring pins holding the existing tubing onto the MagWash aspirator(s).
6. Twist and pull upwards to remove the tubing.

   

7. If the tubing cannot be easily removed from the aspirators, one may use a set of needle-nose pliers to help pull the spring-clip free.

   Note—Take care not to bend or crush the metal aspirator within the tubing during removal.

8. Once removed from the aspirators, take care not to allow any material to drip from the end of the tubing onto any part of the system.
9. Remove the aspirator tubes from the plastic retaining clips along the back of the system as shown below.

   

10. Follow each of the tubes to the vacuum manifold and unscrew each one. Take care not to allow anything inside of the tubing to drip onto the system.

    

11. Remove and dispose of the old tubing in a biohazard waste container.
12. Obtain the new aspiration tubing set. Observe that:
    1. The aspiration tubing spring-clips should be silver in color.
    2. That the spring clips should be pushed approximately 2 mm above the bottom end tubing. (see below)

       

13. Install the new tubing by reversing the steps 5 through 10.

    Note—It is not important which port each tube is screwed into on the vacuum manifold. Each port experiences the same vacuum pressure.

14. Press the new tubing down, fully onto the MagWash aspirators until the tubing abuts the aspirator flange. Do NOT slide the spring clip in relation to the tubing.

    

15. After seating the spring-clip, there should still be 2mm above the bottom of the tubing.
16. Ensure that the new tubing is not tangled and will not interfere with the pipettor arms.

Verification

1. In Service Software, perform a MagWash OQ Test.
   1. Click on the System tab.
   2. Select the Main 2 tab.
   3. Check the Report, Initialize, MagWash #1, and MagWash #2 boxes.
   4. Click on the Mag Wash OQ Test button.

      

2. Follow the on-screen prompts to complete the Mag Wash OQ Test.

   Note—Service Software 0.2.2.0 will always indicate that the Mag Wash OQ Test has failed. This is a software bug and will be updated in future Service Software revisions.

3. Do not look at the overall test results. Instead look at the generated OQ test report. Inspect the test report and make sure that all Tubes/Needles pass for "Wash LLV" and "Residual Check".

   

4. Inspect for any kinks or crushed tubing.
5. Inspect for any leaks at the vacuum fittings.
6. PrimeOperation of pumping fluid through tubing to ensure proper and consistent fluid delivery (remove air from the tubing, etc.). the system.
7. The System should not report any MagWash or Wash Buffer erros.